How to build your reseach?

Solutions to Yeast Genomes

SAturated Transposon Analysis in Yeast (SATAY) is a novel method based on random transposon insertion and next-generation sequencing to functionally screen the genome of Saccharomyces cerevisiae. SATAY can reveal positive and negative genetic interactions, auxotrophies, drug-sensitive or -resistant mutants, and map functional protein domains. SATAY combines advantages from both classical genetics and high-throughput robotic screens. SATAY can in principle be implemented in any strain background, since it does not rely on the existence of available deletion libraries and does not necessitate markers to follow genetic traits.

SATAY yields unprecedented insight on the domain structure-function relationship of important proteins, and allows the mapping of important functional domains, without prior knowledge. SATAY does not require any particular equipment besides access to a sequencing platform, and involves a limited amount of work. It can be easily multiplexed to perform several genome-wide screens simultaneously.

SATAY is particularly suited to discover loci important for growth under various conditions, and it can

• reveal positive and negative genetic interactions in single and multiple mutant strains
• identify drug targets
• detect not only essential genes, but also essential protein domains
• generate both null and other informative alleles

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