Rewiring phospholipid biosynthesis reveals robustness in membrane homeostasis and uncovers lipid regulatory players
Intracellular transport of lipids by Lipid Transport Protein (LTP) works alongside vesicular transport to shuttle lipids from their place of synthesis to their destinations. Whereas many LTPs have been identified, it is largely unknown which routes and which LTPs a given lipid utilizes to navigate the multiple membranes of eukaryotic cells. The major and essential phospholipids, phosphatidylethanolamine (PE) and phosphatidylcholine (PC) can be produced by multiple pathways and, in the case of PE, also at multiple locations. Here, we present an approach in which we simplify and rewire yeast phospholipid synthesis by redirecting PE and PC synthesis reactions to distinct subcellular locations using chimeric enzymes fused to specific organelle targeting motifs. In rewired conditions, viability is expected to depend on homeostatic adaptation to the ensuing lipostatic perturbations and on efficient interorganelle lipid transport. We therefore performed genetic screens to identify factors involved in both of these processes. The set of genes that we identified is enriched for functions linked to transcriptional regulation of lipid homeostasis, lipid metabolism and transport. In particular, we identify a requirement for Csf1 –an uncharacterized protein harboring an N-chorein lipid transport domain- for survival under certain rewired conditions as well as lipidomic adaptation to cold, implicating it in interorganelle lipid transport and homeostatic adaptation.
Transposon mutagenesis screens have been generated either in KennedyON conditions (supplemented with 10 mM ethanolamine and 10 mM choline) or KennedyOFF conditions in the following ‘rewired’ yeast strains:
Pmt in lipid droplets, Psd in lipid droplets (Pmt-LD/Psd-LD)
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